Differential Display Reverse Transcription Polymerase Chain Reaction (DDRT-PCR) for Grey Oyster Mushroom Samples Grown with Acoustic Sound Treatment

Abstract Grey oyster mushroom is the most often farmed and marketed type of for household consumption in Malaysia. The cultivation takes a long time due to its complicated mycelium growth. Several treatments were introduced such as acoustic sound treatment increase growth rate quality crop, but there no in-depth study regarding genetic expression genes affected. This aims evaluate Differential Display Reverse Transcriptase-Polymerase Chain Reaction (DDRT-PCR) grey samples grown with treatment. was subjected RNA extracted from converted into cDNA before undergoing DDRT-PCR. Ten Expressed Transcripts (DETs) that successfully identified based on differences between intensity absence amplicons sent gene sequencing BLAST through NCBI database obtain relevant results possible annotation. Seven out ten DETs hit potential encoding housekeeping structural development functions. showed did affect certain differently captured by DDRT-PCR analyses offers new ideas ecological agriculture.